Peter Parker / Spider-Man (Spidey) (voice) (136 Episodes)

Gwen Stacy / Ghost-Spider (voice) (136 Episodes)

Miles Morales / Spider-Man (Spin) (voice) (136 Episodes)

Norman Osborn / Green Goblin (voice) (36 Episodes)

Carolyn Trainer / Doctor Octopus (voice) (31 Episodes)

Aunt May (voice) (23 Episodes)

Aleksei Sytsevich / Rhino (voice) (20 Episodes)

Francine Frye / Electro (voice) (12 Episodes)

Bruce Banner / Hulk (voice) (12 Episodes)

Trace-E (voice) (7 Episodes)

T'Challa / Black Panther (voice) (8 Episodes)

Kamala Khan / Ms. Marvel (voice) (8 Episodes)

Felicia Hardy / Black Cat (voice) (7 Episodes)

Mr. Von Carnegie (voice) (7 Episodes)

Rio Morales (voice) (6 Episodes)

Helen Stacy (voice) (6 Episodes)

Tony Stark / Iron Man (voice) (6 Episodes)

Jefferson Morales (voice) (6 Episodes)

William Baker / Sandman (voice) (5 Episodes)

George Stacy (voice) (4 Episodes)

Hank Pym / Ant-Man (voice) (3 Episodes)

Janet Van Dyne / Wasp (voice) (3 Episodes)

Humberto Lopez / Reptil (voice) (3 Episodes)

Isla Coralton (voice) (1 Episodes)

Gloria Morales (voice) (1 Episodes)

Mayor (voice) (1 Episodes)

Concert Announcer (voice) (1 Episodes)

Jerry (voice) (1 Episodes)