Peter Parker / Spider-Man (voice) (103 Episodes)

Aunt May Parker (voice) (103 Episodes)

J. Jonah Jameson (voice) (103 Episodes)

Luke Cage / Power Man (voice) (103 Episodes)

Nick Fury (voice) (78 Episodes)

Prowler (voice) (52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

Phil Coulson (voice) (52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

Ava Ayala / White Tiger (voice) (52 Episodes)

Dr. Otto Octavius / Bentley Wittman (voice) (52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

(52 Episodes)

Flash Thompson (voice) (52 Episodes)

Danny Rand / Iron Fist (voice) (52 Episodes)

Harry Osborn (voice) (52 Episodes)

Sam Alexander / Nova (voice) (52 Episodes)

Arnim Zola (voice) (5 Episodes)

Dagger (voice) (6 Episodes)

Spider-Man Noir (voice) (4 Episodes)

Bone Spider/Goliath Spider (voice) (2 Episodes)

Jack Russell / Werewolf by Night (voice) (1 Episodes)

Captain Ultra/Piledriver (voice) (1 Episodes)

Korvac/Chitauri #3 (voice) (1 Episodes)

Rocket Raccoon (voice) (1 Episodes)

Captain George Stacy (voice) (1 Episodes)

Alex O'Hirn / Rhino (voice) (1 Episodes)

Wolverine (voice) (1 Episodes)

Sabretooth/Construction Worker/Announcer (voice) (1 Episodes)

Scott Lang / Ant-Man (voice) (1 Episodes)

Jessie Prescott (voice) (1 Episodes)

Emma Ross (voice) (1 Episodes)

Luke Ross (voice) (1 Episodes)

Ravi Ross (voice) (1 Episodes)

Zuri Ross (voice) (1 Episodes)

Rick Jones / A-Bombardier (voice) (1 Episodes)

Gwen Stacy, Spider-Gwen (voice) (1 Episodes)

Wolf Spider, Spyder-Knight (voice) (1 Episodes)

Madame Web (voice) (1 Episodes)

Blood Spider (voice) (1 Episodes)