Max Modell (voice) (57 Episodes)

Anya Corazon (voice) (57 Episodes)

Flash Thompson (57 Episodes)

Aunt May (voice) (57 Episodes)

Harry Osborn (voice) (57 Episodes)

Gwen Stacy (voice) (57 Episodes)

Norman Osborn (voice) (57 Episodes)

Miles Morales (voice) (57 Episodes)

Raymond Warren (voice) (57 Episodes)

Peter Parker / Spider-Man (voice) (57 Episodes)

Doc Ock / Otto Octavius (voice) (57 Episodes)

Adrian Toomes (voice) (57 Episodes)

Uncle Ben (voice) (57 Episodes)

Hammerhead (voice) (57 Episodes)

Alistair Smythe (voice) (3 Episodes)

Tinkerer (voice) (2 Episodes)

Sand Girl (voice) (2 Episodes)

Nocturnal (voice) (1 Episodes)

J. Jonah Jameson (voice) (3 Episodes)

Randy Robertson (voice) (2 Episodes)

Aleksei Sytsevich/Comic Book Guy (voice) (1 Episodes)

Black Cat/Nurse/Mom (voice) (1 Episodes)

Herman Schultz / Shocker (voice) (1 Episodes)

Prowler (voice) (1 Episodes)

Police Officer #3 (voice) (1 Episodes)

Cop (voice) (1 Episodes)

Sandman / Flint Marko (voice) (1 Episodes)

Ross Caliban (voice) (1 Episodes)

Yuri Watanabe (voice) (1 Episodes)

Mary Jane (voice) (1 Episodes)

Swarm (voice) (1 Episodes)

Head Administrator (voice) (1 Episodes)