Asphalt Jack returns to Chicago to visit Charley and friends with his fiancé, but the visit turns sour when Jack and his new cyber bike are captured and the guys must rescue him while Limburger and Brie deal with a quadruple, triple, double agent who has deals with more then just the two Plutarkians.
Limburger uses Karbunkle's newest machine to steal the land from fictional places in Shakespeare's plays. This resulted in the destruction of the plays, and they ceased to exist. So, the Biker Mice must enter the well-known stories to stop him, but first Charley must convince them that Shakespeare is worth saving.