Bighead and his henchmen blast Ace and Gary with a flesh ray, transforming them from animated characters to live-action ones, in which they are portrayed by Jon Hamm and Jimmy Fallon, respectively. The gun malfunctions and "unanimates" everyone, with Ed Helms playing Half-Scary, a Two-Face-like henchman, Fred Armisen as Lizardo, Stephen Colbert as Dr. Brainio, and Steve Carell as Bighead.

Bighead enlists a Minneapolis-St. Paul Airport undercover policeman at a BBQ he orchestrates to out Ace and Gary, with surprising results.

Bighead is at it again, and redecorates his lair in another attempt to out Gary and Ace. The Duo is assisted by former GE chairman, and superhero, Jack Welch.

Ace and Gary battle Bighead's evil schemes at the NBA Finals.

Even before they were the Ambiguously Gay Duo, teenagers Ace and Gary fought evil. This episode shows that Bighead was in their class, and he is determined to win his classmates' respect and "out" the duo. When he reanimates and enlarges a giant frog, the AmbiguoBoys must stop him.

Ace and Gary are oblivious to the suggestiveness in letters from their fans, who are mostly criminal convicts.

Ace and Gary question their origins as superheroes and why everybody regards them strangely at the Fortress of Privacy.

The Duo battles Dr. Brainio and Bighead's ice monster creation.

Ace and Gary demonstrate bicycle and home safety tips for local kids.

Santa Claus has been kidnapped by aliens, and the Duo must save him.

Ace and Gary stop Bighead and Queen Serena's evil scheme.

Ace and Gary foil Bighead's plan to take over Metroville.